Postdoctoral Researcher – Genome Editing for Genetic Skin Diseases (F/M) Institut Imagine

About the position

The laboratory “Genetic Skin Diseases: from Pathophysiological Mechanisms to Treatments”, led by Prof. Alain Hovnanian at the Imagine Institute (INSERM UMR 1163), is seeking a highly motivated Postdoctoral Researcher to develop innovative genome editing strategies for genetic skin diseases. The position will be based in the Gene Editing subgroup under the supervision of Dr. Araksya Izmiryan.

https://www.institutimagine.org/en/alain-hovnanian-182

The postdoctoral researcher will contribute to an ANR-funded research project focused on the development of therapeutic genome editing approaches for epidermolysis bullosa. The project combines base editing and prime editing with patient-derived primary, induced pluripotent stem cells (iPSCs), and advanced skin organoid models to investigate and validate mutation-specific correction strategies.

Research project

The project aims to develop and evaluate precise genome editing approaches in clinically relevant human cellular and tissue models. A particular focus will be placed on editing patient-derived cells, followed by functional validation in 3D skin models and skin organoids. The project also incorporates single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics to provide high-resolution molecular and spatial characterization of patient-derived skin organoids and edited skin models.

Main responsibilities

• Design, implement and optimize base editing and prime editing strategies;

• Design and characterize guide RNAs, including assessment of editing efficiency, specificity and potential toxicity;

• Develop and evaluate delivery strategies, including lipid nanoparticles (LNPs);

• Perform genome editing in human keratinocytes, fibroblasts and patient-derived RDEB iPSCs;

• Culture and maintain human iPSCs and and contribute to their differentiation into relevant skin cell populations;

• Develop, culture and characterize patient-derived skin organoids and 3D skin equivalents for disease modelling and functional validation;

• Perform molecular and cellular biology analyses, including DNA/RNA/protein extraction, PCR, RT-PCR, Sanger and NGS sequencing, FACS, immunocytochemistry and immunohistochemistry;

• Assess correction at the molecular and functional levels, including dermal–epidermal junction integrity and collagen VII expression in relevant epidermolysis bullosa models;

• Contribute to single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics analyses to characterize edited cells and skin organoids, assess cellular states and differentiation, and investigate molecular and spatial responses to genome correction;

• Analyze and interpret experimental data and contribute to scientific presentations, manuscripts and project reporting.

Training can be provided for specific advanced techniques when required.