Le contenu du poste est libellé en anglais car il nécessite de nombreuses interactions avec nos filiales à l’international, l'anglais étant la langue de travail.

About the Opportunity 

As an experienced mRNA Post Doctoral - mRNA Scientist, you will be in charge of designing, characterizing and optimizing mRNA vaccine production from A to Z.

In the race for the future of health – The Sanofi MRNA Center of Excellence

At Sanofi, we believe in a world in which no one suffers or dies from vaccine-preventable diseases. And while the pandemic has shaken up our industry, creating new opportunities and risks, we believe the acceleration of innovation with mRNA technology means vaccines for more people faster than before. That’s why we’re looking for bold, optimistic world-changers to join the team at our mRNA Center of Excellence. We’re investing €400m a year into the new Center, hiring a team of 400 dedicated employees for it and using it as the foundation to implement integrated end-to-end mRNA vaccine capabilities and research with R&D, digital and CMC teams.

Bring your ambition and optimism for the future to Sanofi. Join us

OVERALL RESPONSIBILITY

• To lead Correlative Light and Electron Microscopy (CLEM) method development at PRD level and mRNA Center to support mRNA projects

OVERALL COMPETENCY

• Experienced in Photonic and ultrastructural Bio-Imagery with proven competencies in super resolution microscopy and correlative light and electron microscopy

ROLE DESCRIPTION & RESPONSIBILITIES

• Develop CLEM solution for particle analysis of mRNA vaccine projects to characterize LNP-mRNA particles and elucidate mRNA cell delivery
• Participate to mRNA method development and analysis
• Provide a strong support for cell-based assay development and analysis for LNP characterization
• Design protocol for CLEM by STORM and STEM-in-SEM
• Two-colour super-resolution imaging, with ONI Nanoimager
• Develop bioinformatics correlative analysis and data interpretation
• Set up tools to support LNP-mRNA formulation
• Work in a transdisciplinary environment
• Mobility (work between two SP sites around Lyon)

RELEVANT QUALIFICATIONS & CAPABILITIES

PhD in CLEM/image processing analysis/biology/data management with at least 3 years at level of Research laboratory

Knowledge and Skills – Required

• Solid experiences in CLEM
• Solid experience in fluorescence microscopy
• Solid experience in electron microscopy, resin embedding and ultramicrotomy
• Used to work closely with biologists
• Image processing and analysis software such as ImageJ, Fuji, Zen
• Programming languages such as Java and Python
• Excellent verbal and written communication skills are required

Knowledge and Skills – Preferred

• Experience in mRNA, LNP, vesicles
• Experience in super resolution microscopy, STORM, PALM, TIRF new dye (SNAP TAG, DNA paint)
• Experience in SEM or/and TEM equipped with STEM
• Ability to work in a team
• Perfect English language
• Experience in confocal microscopy andparticle-tracking would be a plus
• Experience in vaccine projects and microbiology would be a plus
• Experience with new fluorescent probes for mRNA and lipids will be appreciated

#MRNA

At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.